Gst gene fusion system manual

User Manual Version Product name: Glutathione Agarose Beads Cat #: GAB, GAB, GAB, GAB-OEM Description: Glutathione S-transferase (GST) gene fusion systems have been widely used for obtaining large amounts of desirable protein in expressed Escherichia coli. The fusion proteins, which contain a GST tail can then be. User Manual BaculoDirect™ GST Gateway® Expression Kit For cloning and high-level expression of recombinant GST-fusion proteins using Gateway®-adapted Baculovirus DNA Catalog nos. A, A Rev. Date: 14 July Manual part no. A MAN Data file AA GST Gene Fusion System Glutathione S-transferase (GST) Gene Fusion System The Glutathione S-transferase (GST) Gene Fusion System from GE Healthcare is a versatile system for the expression, purification, and detection of GST-tagged proteins produced in E. coli. The system consists of three major components.

The Glutathione S-transferase (GST) Gene Fusion System is an integrated system for the expression, purification and detection of fusion proteins produced in E. coli. The system consists of three major components: pGEX plasmid vectors*, two GST Purification Modules and the GST Detection Module. A series of site-specific proteases complements the system. GST is a amino acid protein (26 kDa) whose DNA sequence is frequently integrated into expression vectors for production of recombinant proteins. The result of expression from this vector is a GST-tagged fusion protein in which the functional GST protein (26 kDa) is fused to the N-terminus of the recombinant protein. Gene Fusion Expression Systems Glutathione S-transferase (GST) pGEX Vectors*(GST Gene fusion) All of the GST gene fusion vectors offer: • A tac promoter for chemically inducible, high-level expression. Map of the glutathione S-transferase fusion vectors showing the reading frames and main features.

pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression. Components: pET. First and foremost, when expressed in bacteria alone, or as a fusion, GST is not sequestered in inclusion bodies (in contrast to previous fusion protein systems). Bacteria are widely used for His or GST tagged recombinant protein expression. Shin SI, GST Gene Fusion System Handbook, Amersham Biosciences, Code No.